Evaluating HER2 Gene Amplification Using Chromogenic In Situ Hybridization (CISH) Method In Comparison To Immunohistochemistry Method in Breast Carcinoma
نویسندگان
چکیده
منابع مشابه
Predicting Effects of Clinicopathological Variables on Her2 Gene Amplification by Chromogenic in situ Hybridization (CISH) in IHC Her2 (2+) Breast Cancer Patients; A Study from Iran
Background & Objective: The her2 amplification plays an important role in breast cancer management. Therefore, there is a need for using supplementary molecular methods in IHC equivocal cases. Present study has been conducted to determine the effects of clinicopathological variables on her2 gene amplification by chromogenic in situ hybridization (CISH) in IHC ...
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Background: Breast cancer is the most common malignancy among women worldwide.The human epidermal growth factor receptor (HER) is a transmembrane receptor in the epidermal growth factor (EGF) receptor family located at the long arm of human chromosome 17 (17q12). Diagnostic assays for HER2 in breast cancer provide important prognostic information and independently help guide management by ident...
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Background and objective: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ Hybridization (CISH) to assess HER2 status of biopsy specimens. <...
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Methods: MYC gene amplification by FISH and DAKO dual-color CISH Results: The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals. The dual –color CISH assay was performed on 40 cases of prostate cancer. Amplification was identified in 12 of 40 (30%) tumors. No amplification was seen in 28 of 40 (70%) tumors. F...
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One of the prognostic and predictive factors in invasive breast carcinomas is determination of the HER2/neu proto-oncogene amplification or HER2 protein overexpression. HER2 amplification/overexpression is associated with a more aggressive disease course, greater likelihood of recurrence and generally poor prognosis. The authors compared the specificity, simplicity of a given procedure and meth...
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ژورنال
عنوان ژورنال: Open Access Macedonian Journal of Medical Sciences
سال: 2018
ISSN: 1857-9655
DOI: 10.3889/oamjms.2018.455